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KMID : 0613820120220111507
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Journal of Life Science
2012 Volume.22 No. 11 p.1507 ~ p.1514
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Antioxidative Activity of Cornus officianalis Extracts Obtained by Four Different Extraction Techniques
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Park Eun-Bi
Kim Hye-Sun
Shin So-Yun
Ji In-Ae
Kim Ji-Hyun
Kim Sung-Goo
Yoo Byung-Hong
Kim Byung-Woo
Kwak In-Seok
Kim Moon-Moo
Chung Kyung-Tae
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Abstract
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Oxidative stress leads to damage in all components of the cell, including proteins, lipids, and DNA due to imbalance between reactive oxygen species production and cellular detoxification. Phytochemicals are well-known to contain antioxidants, and their physiological role has been intensively studied. The fruit of Cornus officianalis has been used in oriental medicine and has been reported to have many functions. In this study, four different extraction techniques were applied to extract functional components from the fruit of Cornus officianalis, and the content of loganin, which is an antioxidant having DPPH radical and hydrogen peroxide scavenging activity and reducing power, was analyzed in each extract. Extraction techniques employed in this study were heat extraction by water, 70% ethanol extraction, enzyme treatment, and combination of enzyme treatment and heat extraction by water. All extracts contained 11.8-18.0 mg/g loganin and showed antioxidation function assayed by measuring DPPH radical and hydrogen peroxide scavenging activity and reducing power. Among them, heat extraction was the most effective technique, showing a maximum amount of loganin (18.0 mg/g) and antioxidative activity at 100 mg/ml concentration. Each extract showed very low cytotoxicity up to at 500 mg/ml but 10-20% cytotoxicity at 1,000 mg/ml by in vitro MTT assay.
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KEYWORD
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Cornus officianalis, extraction techniques, loganin, antioxidant, DPPH radical
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